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Product Name
TRIM14 antibody
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Description
TRIM14 Rabbit Polyclonal antibody. Positive WB detected in L02 cells. Positive IF detected in Hela. Positive IHC detected in human liver. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human; other species not tested.
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Alternative names
TRIM14 antibody; tripartite motif containing 14 antibody; KIAA0129 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM14 recombinant protein (Accession Number: XM_047424162). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:116298(TRIM14 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:116298(TRIM14 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:116298(TRIM14 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TRIM14 antibody Catalog No:116298 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"