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Product Name
TRAP1 antibody
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Description
TRAP1 Rabbit Polyclonal antibody. Positive WB detected in Raji cells, HeLa cells, HepG2 cells, HL-60 cells, K-562 cells, mouse heart tissue, NIH/3T3 cells. Positive IF detected in Hela cells. Positive IHC detected in human heart tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 66-70 kDa ,60 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HSP 75 antibody; HSP75 antibody; HSP90L antibody; TNFR assOCiated protein 1 antibody; TRAP 1 antibody; TRAP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRAP1 recombinant protein (Accession Number: BC000406). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:116338(TRAP1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:116338(TRAP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:116338(TRAP1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TRAP1 antibody Catalog No:116338 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HeLa cells were stained with 0.2ug TRAP1 antibody (Catalog No:116338, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the hsp90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in hsp75, but not in other hsp90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"