Transportan peptide

Transportan peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-GWTLNSAGYLLGKINLKALAALAKKIL-OH

H-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Lys-Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-OH

27

95.22%

C₁₃₄H₂₂₆N₃₄O₃₃

2841.42

Synthetic

Powder

Transportan is a 27 amino acid chimeric peptide consisting of 12 amino acids from the amino terminal of galanin and the 14 amino acid peptide mastoparan connected via a lysine residue. Transportan possesses characteristics of both galanin and mastoparan as it inhibits the binding of galanin to the GALR-1 receptor as well as modulating the activity of G proteins through inhibition of GTPase. Transportan is a cell-penetrating peptide which can penetrate into many cell types in a rapid and efficient way. In the cell interior, Transportan localizes mostly in membranous structures and is conveyed into the nuclei, where it concentrates in the nucleoli.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Pooga et al (1998) Cell penetration by transportan. FASEB J. 12(1) 67 PMID: 9438412
• Pooga et al (2001), Cellular translocation of proteins by transportan. FASEB J. 5 1451 PMID: 11387254
• Aguiar etal (2019) Coupling the cell-penetrating peptides transportan and transportan 10 to primaquine enhances its activity against liver-stage malaria parasites. Med. Chem. Commun. 10 221 PMID: 30881610

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.