Product Name

TRAIL antibody

Documents

Description

TRAIL Rabbit Polyclonal antibody. Positive IP detected in A549 cells. Positive WB detected in MCF7 cells, A549 cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 20 kDa

Tested applications

ELISA, WB, IP, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

Apo 2 ligand antibody; Apo 2L antibody; APO2L antibody; CD253 antibody; Protein TRAIL antibody; TL2 antibody; TNFSF10 antibody; TRAIL antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of TRAIL recombinant protein (Accession Number: NM_003810). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IF: 1:20-1:200

Validations

MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116236(TRAIL antibody) at dilution of 1:1000

IP Result of anti-TRAIL (IP:Catalog No:116236, 4ug; Detection:Catalog No:116236 1:700) with A549 cells lysate 1600ug.

Immunofluorescent analysis of (-20oc Ethanol) fixed MCF-7 cells using Catalog No:116236(TRAIL Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

TRAIL is a 30 kD protein known as TNF-related apoptosis inducing ligand, CD253, TNFSF10, or Apo-2L. It is expressed as two kinds of forms: cell surface protein (30 kD) and soluble ligand (19-20 kD) produced by megakaryocytes and platelets, monocytes, neutrophils, NK cells and activated T cells. This antigen is also expressed on tumor cells and transformed cell lines. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process.

References

Zhang J, Huang S, Zhang H. Targeted knockdown of Bcl2 in tumor cells using a synthetic TRAIL 3'-UTR microRNA. International journal of cancer. 126(9):2229-39. 2010.
Liu L, Su Z, Xin S. The calcineurin B subunit (CnB) is a new ligand of integrin αM that mediates CnB-induced Apo2L/TRAIL expression in macrophages. Journal of immunology (Baltimore, Md. : 1950). 188(1):238-47. 2012.
Su Z, Xin S, Xu L. The calcineurin B subunit induces TNF-related apoptosis-inducing ligand (TRAIL) expression via CD11b-NF-κB pathway in RAW264.7 macrophages. Biochemical and biophysical research communications. 417(2):777-83. 2012.
Li HY, Zhang J, Sun LL. Celastrol induces apoptosis and autophagy via the ROS/JNK signaling pathway in human osteosarcoma cells: an in vitro and in vivo study. Cell death & disease. 6:e1604. 2015.

TRAIL antibody

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