Cart 2

TOP3A antibody

Cat.#: 116249

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    TOP3A antibody
  • Documents
  • Description
    TOP3A Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, HL-60 cells. Observed molecular weight by Western-blot: 100-110 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DNA topoisomerase 3 alpha antibody; DNA topoisomerase III alpha antibody; TOP3 antibody; TOP3A antibody; topoisomerase (DNA) III alpha antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TOP3A recombinant protein (Accession Number: NM_004618). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:116249(TOP3A antibody) at dilution of 1:1000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:116249(TOP3A antibody) at dilution of 1:1000

    IP Result of anti-TOP3A (IP:Catalog No:116249, 4ug; Detection:Catalog No:116249 1:500) with K-562 cells lysate 3600ug.

    IP Result of anti-TOP3A (IP:Catalog No:116249, 4ug; Detection:Catalog No:116249 1:500) with K-562 cells lysate 3600ug.

  • Background
    DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110kd human TOP3A.
  • References
    • Chaudhury I, Sareen A, Raghunandan M, Sobeck A. FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery. Nucleic acids research. 41(13):6444-59. 2013.
    • Chaudhury I, Stroik DR, Sobeck A. FANCD2-controlled chromatin access of the Fanconi-associated nuclease FAN1 is crucial for the recovery of stalled replication forks. Molecular and cellular biology. 34(21):3939-54. 2014.
    • Blackford AN, Nieminuszczy J, Schwab RA, Galanty Y, Jackson SP, Niedzwiedz W. TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation. Molecular cell. 57(6):1133-41. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"