Human TLR4 / TLR-4 (His Tag) recombinant protein

Human TLR4 / TLR-4 (His Tag) recombinant protein

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Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.

Toll-like receptor 4

LPS; CD284; RAN/M1; RP24-209H15.1; RASL2-8; TLR-4; LY87; ARMD10; TOLL

O00206

TLR4

Baculovirus-Insect Cells

A DNA sequence encoding the human TLR4 (Met 1-Lys631) (O00206-1) was expressed, with a C-terminal polyhistidine tag.

Human

The secreted recombinant human TLR4 consists of 619 amino acids and predicts a molecular mass of 70.5 KDa. The apparent molecular mass of the protein is approximately 68 Kda in SDS-PAGE under reducing conditions due to glycosylation.

> 87 % as determined by SDS-PAGE