TMTP1 peptide

TMTP1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-NVVRQC-OH

H-Asn-Val-Val-Arg-Gln-Cys-OH

6

95.46%

C₂₈H₅₁N₁₁O₉S

717.83

Synthetic

Powder

The TMTP1 polypeptide is the five amino acid sequence NVVRQ identified by using the bacterial flagellin library display technology. TMTP1 could target the primary highly metastatic tumor and the micrometastases. TMTP1 also specifically recognized atypical liver micrometastases that contained infrequent neoplastic cells. Binding peptides and fusion proteins prepared by combining TMTP1 with apoptotic peptides or diphtheria toxin could specifically inhibit tumor metastasis. XPNPEP2, a subtype of aminopeptidase P, is the potential receptor of TMTP1. XPNPEP2 expression was obviously upregulated in CC tissue and that overexpression of XPNPEP2 was correlated with lymph node metastasis and worse overall survival. TMTP1 polypeptide can be used to develop new diagnostic agents for the CC metastasis, especially micrometastasis.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wei R, Jiang G, Lv M, Tan S, Wang X, Zhou Y, Cheng T, Gao X, Chen X, Wang W, Zou C, Li F, Ma X, Hu J, Ma D, Luo D, Xi L. TMTP1-modified Indocyanine Green-loaded Polymeric Micelles for Targeted Imaging of Cervical Cancer and Metastasis Sentinel Lymph Node in vivo. Theranostics. 2019 Sep 25;9(24):7325-7344. doi: 10.7150/thno.35346. PMID: 31695771; PMCID: PMC6831285.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.