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Product Name
Human TFPI2 (His Tag) recombinant protein
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Description
May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.
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Protein name
Tissue factor pathway inhibitor 2
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Protein short names
REF1; PP5/TFPI-2; TFPI-2; TFPI2; FLJ21164  PP5; AV000670
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Uniprot ID
P48307
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Gene Name
TFPI2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.
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Validations
Human TFPI2 Protein (His Tag) SDS-PAGE
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