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Product Name
TIMM50 antibody
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Description
TIMM50 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human liver tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, rat kidney tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 35-40 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
TIM50 antibody; TIM50L antibody; TIMM50 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TIMM50 recombinant protein (Accession Number: BC121147). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116134(TIMM50 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:116134(TIMM50 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:116134(TIMM50 Antibody) at dilution of 1:50
IP Result of anti-TIMM50 (IP:Catalog No:116134, 4ug; Detection:Catalog No:116134 1:1000) with HeLa cells lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:116134(TIMM50 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TIMM50 represents translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)
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