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Product Name
Tim23 antibody
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Description
Tim23 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human heart tissue, human testis tissue. Positive IP detected in mouse heart tissue. Positive WB detected in Jurkat cells, COLO 320 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
PRO1197 antibody; TIM23 antibody; TIMM23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Tim23 recombinant protein (Accession Number: BC062707). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116127(TIMM23 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116127(TIMM23 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-Tim23 (IP:Catalog No:116127, 4ug; Detection:Catalog No:116127 1:1000) with mouse heart tissue lysate 3200ug.
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Background
Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.
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References
- Satoh K, Hamada S, Kimura K. Up-regulation of MSX2 enhances the malignant phenotype and is associated with twist 1 expression in human pancreatic cancer cells. The American journal of pathology. 172(4):926-39. 2008.
- Tang G, Gutierrez Rios P, Kuo SH. Mitochondrial abnormalities in temporal lobe of autistic brain. Neurobiology of disease. 54:349-61. 2013.
- Huang R, Zhao L, Chen H. Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV. PloS one. 9(5):e96246. 2014.
- Zhang X, Zuo X, Yang B. MicroRNA directly enhances mitochondrial translation during muscle differentiation. Cell. 158(3):607-19. 2014.
- Murakawa T, Yamaguchi O, Hashimoto A. Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation. Nature communications. 6:7527. 2015.
- Ding C, Wu Z, Huang L. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Scientific reports. 5:16064. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"