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Product Name
TICAM2 antibody
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Description
TICAM2 Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, HeLa cells, human testis tissue. Positive FC detected in HeLa cells. Positive IHC detected in human testis tissue, human prostate hyperplasia tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human; other species not tested.
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Alternative names
TICAM 2 antibody; TICAM2 antibody; TIRAP3 antibody; TIRP antibody; TRAM antibody; TRIF related adapter molecule antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_021649). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116120(TICAM2 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116120(TICAM2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116120(TICAM2 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug TICAM2 antibody (Catalog No:116120, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
TICAM2, also named as TIRAP3, TIRP and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.In WB, the MW of TICAM2 is about 27-32kd.
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