Tet213 peptide

Tet213 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

1 Vial

H-KRWWKWWRRC-OH

H-Lys-Arg-Trp-Trp-Lys-Trp-Trp-Arg-Arg-Cys-OH

10

95.98

C₇₇H₁₀₇N₂₅O₁₁S

1590.89

Synthetic

Coatings containing the peptide Tet213 (KRWWKWWRRC) demonstrated antimicrobial activity against S. aureus and P. aeruginosa bacterial strains while remaining cytocompatible towards MG-63 osteoblast-like cells. A similar coating loaded with HHC36 (KRWWKWWRR) did not interfere with bone growth around press-fit grafts in a rabbit femoral defect model.

Ideally Tet213 peptide should be stored in a freezer at or below -9C. Tet213 peptide should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Kazemzadeh-Narbat M, Kindrachuk J, Duan K, Jenssen H, Hancock REW, Wang R (2010) Antimicrobial peptides on calcium phosphate-coated titanium for the prevention of implant-associated infections. Biomaterials 31:9519–9526. https://doi.org/10.1016/j. biomaterials.2010.08.035

Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

TFA Removal Service is recommended for:

• Peptides that will be used in cellular assays
• Peptides that will be used as APIs or in manufactured products
• For hydrophilic peptides containing numerous basic residues