Tet1 peptide (TFA removed)

Tet1 peptide (TFA removed)

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-HLNILSTLWKYR-OH

H-His-Leu-Asn-Ile-Leu-Ser-Thr-Leu-Trp-Lys-Tyr-Arg-OH

12

95.46%

C₇₃H₁₁₄N₂₀O₁₇

1543.8

Synthetic

Powder

Tet1 (HLNILSTLWKYR), a peptide identified by in vitro phage display, binds to GT1B gangliosides, sphingophospholipids highly expressed on neuronal cell types. Tet1 has the binding characteristics of tetanus toxin, can interact specifically with motor neurons, and is capable of retrograde delivery in the neuronal tissues due to its affinity to ganglioside GT1B receptor.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Mathew, A.; Fukuda, T.; Nagaoka, Y.; Hasumura, T.; Morimoto, H.; Yoshida, Y.; Maekawa, T.; Venugopal, K.; Kumar, D.S. Curcumin loaded-PLGA nanoparticles conjugated with Tet-1 peptide for potential use in Alzheimer's disease. PLoS ONE 2012, 7, e32616.
• Jia, T.T.; Sun, Z.G.; Lu, Y.; Gao, J.; Zou, H.; Xie, F.Y.; Zhang, G.Q.; Xu, H.; Sun, D.X.; Yu, Y.; et al. A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-β1-42-injected mice. Int. J. Nanomed. 2016, 11, 3765-3775.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.