Human Cripto/TDGF1/ CRGF (His Tag) recombinant protein
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Teratocarcinoma-derived growth factor 1
Protein short names
CR1; CRIPTO; CRGF; CR; TDGF1
A DNA sequence encoding the human TDGF1 (AAH22393.1) (Met 1-Thr 172) with a C-terminal polyhistidine tag was expressed.
The secreted recombinant human TDGF1 comprises 153 amino acids with a predicted molecular mass of 17.4 kDa. As a result of glycosylation, the apparent molecular mass of rhTDGF1 is approximately 25-30 kDa in SDS-PAGE under reducing conditions.
> 95 % as determined by SDS-PAGE
Measured by its binding ability in a functional ELISA. Immobilized human TDGF1 at 2 μg/ml (100 μl/well) can bind human ALK4 with a linear range of 0.0068-0.16 μg/ml.
Human Cripto / TDGF1 Protein (His Tag) SDS-PAGE
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