Temporin-CPa peptide

Temporin-CPa peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

IPPFIKKVLTTVF

Ile-Pro-Pro-Phe-Ile-Lys-Lys-Val-Leu-Thr-Thr-Val-Phe

13

96%

Synthetic

Powder

For this series of peptides, there were not enough material for antimicrobial assays. However, synthetic temporin-CPa showed activity against E. coli (MIC 25 uM), S. aureus (50 uM), and C. albicans (25 uM). It displayed hemolytic activity (human red blood cells) at LC50=220 uM. Temporin-CPa has Antibacterial, Antifungal activity. The source of Temporin-CPa is the New World frog Lithobates capito, North America.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Conlon JM, Meetani MA, Coquet L, et al. Antimicrobial peptides from the skin secretions of the New World frogs Lithobates capito and Lithobates warszewitschii (Ranidae). Peptides. 2009;30(10):1775-81.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.