Temporin-1P peptide

Temporin-1P peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FLPIVGKLLSGLL

H-Phe-Leu-Pro-Ile-Val-Gly-Lys-Leu-Leu-Ser-Gly-Leu-Leu-NH2

13

97.3%

C₆₈H₁₁₇N₁₅O₁₄

1368.7

Synthetic

Powder

Active against S. aureus (MIC 110 uM). It is also found in North American frog Rana cascadae and named as temporin-1CSa (Conlon et al 2007 Peptides 28: 1268-1274). It is also found in the mountain yellow-legged frog Rana muscosa and named as temporin-1M (Rollins-Smith LA et al. 2002 Dev Comp Immunol 26:63-72). Therefore, the same peptide has been found in multiple species. Updated 3/2015. Temporin-1P has Antimicrobial activity. The source of Temporin-1P is Rana pipiens, Rana cascadae, or Rana muscosa, North America.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Goraya J, Wang Y, Li Z, et al. Peptides with antimicrobial activity from four different families isolated from the skins of the North American frogs Rana luteiventris, Rana berlandieri and Rana pipiens. Eur J Biochem. 2000;267(3):894-900.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.