TAT-obestatin peptide

TAT-obestatin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-RKKRRQRRRFNAPFDVGIKLSGVQYQQHSQAL-NH2

H-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Phe-Asn-Ala-Pro-Phe-Asp-Val-Gly-Ile-Lys-Leu-Ser-Gly-Val-Gln-Tyr-Gln-Gln-His-Ser-Gln-Ala-Leu-NH2

32

95.88%

C₁₆₉H₂₈₀N₆₂O₄₃

3868.4

Synthetic

Powder

Obestatin is a 23-amino-acid peptide originally regarded as an anorexigenic factor. Obestatin is incapable of crossing the blood brain barrier (BBB), which may affect its biological function. TAT-obestatin is composed of TAT (is capable of delivering different types of proteins through the BBB.) and obestatin. TAT-obestatin might play a role in white adipose tissue metabolism, but its physiological effects on food intake and body weight gain regulation remain unclear.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Ren G, He Z, Cong P, Chen H, Guo Y, Yu J, Liu Z, Ji Q, Song Z, Chen Y. Peripheral administration of TAT-obestatin can influence the expression of liporegulatory genes but fails to affect food intake in mice. Peptides. 2013 Apr;42:8-14. doi: 10.1016/j.peptides.2013.01.004. Epub 2013 Jan 9. PMID: 23313740.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.