TAT-AKAP79 (326-336) peptide

TAT-AKAP79 (326-336) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-YGRKKRRQRRRKSKSEESKRME-OH

H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Lys-Ser-Lys-Ser-Glu-Glu-Ser-Lys-Arg-Met-Glu-OH

22

95.13%

C₁₁₇H₂₁₁N₄₉O₃₄S

2880.29

Synthetic

Powder

TAT-AKAP79 (326-336) is amino acids 326–336 of A-kinase anchoring protein 79 (AKAP79), the human anchoring protein, fused to TAT to promote uptake across the cell membrane. AKAP79 (326-336) is the the key region of AKAP79 for interaction with transient receptor potential vanilloid 1 (TRPV1), the thermo-TRP ion channel activated by painful levels of heat, and AKAP79 binding to TRPV1 blocks TRPV1 sensitization. TAT-AKAP79 (326-336) inhibits inflammatory hyperalgesia in mice.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Btesh et al (2014) Mapping the Binding Site of TRPV1 on AKAP79: Implications for Inflammatory Hyperalgesia. J Neurosci. 33(21) 9184 PMID: 23699529

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.