Tat (48-60) peptide
Not For Human Use, Lab Use Only.
Cat.#: 319090
Special Price 117.7 USD
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Product Name
Tat (48-60) peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-GRKKRRQRRRPPQ-OH
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Sequence
H-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-OH
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Length (aa)
13
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Peptide Purity (HPLC)
95.65%
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Molecular Formula
C70H131N35O16
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Molecular Weight
1719.01
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Source
Synthetic
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Form
Powder
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Description
Tat (48 - 60), also known as HIV-1 Tat (48 - 60), is a cell penetrating cationic peptide derived from the N-terminus of the Tat protein, which is a transactivator of the transcription protein present in the human immunodeficiency virus (HIV). Tat (48-60) is an arginine-rich basic peptide which has three domains that function to aid HIV through transactivation, DNA binding and nuclear transport. Tat (48-60) posseses antibacterial activity but is mainly used for the non disruptive transport of exogenous macromolecules into cells.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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References
- Futaki (2005) Membrane-permeable arginine-rich peptides and the translocation mechanisms. Adv. Drug Deliv Rev. 57(4) 547 PMID: 15722163
- Dennison et al (2007) Interactions of cell penetrating peptide Tat with model membranes: A biophysical study. Biochem. Biophys. Res. Commun. 363(1) 178 PMID: 17854767
- Zhu and Shin (2009) Effects of dimerization of the cell-penetrating peptide Tat analog on antimicrobial activity and mechanism of bactericidal action. J. Pept. Sci. 2009 15(5) 345 PMID: 19206074
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"