Tachyplesin-1 peptide

Tachyplesin-1 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

1 Vial

H-RFC*WKVC*YKGIC*FKKC*K-NH2 (C3-C16 and C7-C12 disulfide bonds)

H-Arg-Phe-Cys-Trp-Lys-Val-Cys-Tyr-Lys-Gly-Ile-Cys-Phe-Lys-Lys-Cys-Lys-Amidation (C3-C16 and C7-C12 disulfide bonds)

17

95.2%

C₉₉H₁₅₅N₂₇O₁₈S₄

2135.70

Synthetic

Tachyplesin 1 (TP1; 1) is an antimicrobial peptide extracted from hemocytes of the Japanese horseshoe crab Tachypleus tridentatus. Tachyplesin I displayed a potent activity against marine vibrios, with a MIC of 0.4-0.8 μg/ml against Vibrio P1. It is highly hemolytic against mammalian erythrocytes, which precludes it from further development.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Edwards, Ingrid A et al. “Contribution of Amphipathicity and Hydrophobicity to the Antimicrobial Activity and Cytotoxicity of β-Hairpin Peptides.” ACS infectious diseases vol. 2,6 (2016): 442-450.
• Edwards IA, Elliott AG, Kavanagh AM, Blaskovich MAT, Cooper MA. Structure-Activity and -Toxicity Relationships of the Antimicrobial Peptide Tachyplesin-1. ACS Infect Dis. 2017 Dec 8;3(12):917-926. doi: 10.1021/acsinfecdis.7b00123. Epub 2017 Oct 3. PMID: 28960954.

Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

TFA Removal Service is recommended for:

• Peptides that will be used in cellular assays
• Peptides that will be used as APIs or in manufactured products
• For hydrophilic peptides containing numerous basic residues