Human CD1B & B2M recombinant protein

Human CD1B & B2M recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CD1B contains 1 Ig-like (immunoglobulin-like) domain and belongs to the CD1 family. CD1 family members are transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. During protein synthesis and maturation, they bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1B localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.. It is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. CD1B is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

T-cell surface glycoprotein CD1b

MGC125990; CD1B ANTIGEN; CD1A; B POLYPEPTIDE; DIFFERENTIATION ANTIGEN CD1-ALPHA-3; CD1B MOLECULE; T-CELL SURFACE GLYCOPROTEIN CD1B; CD1B; B POLYPEPTIDE; MGC125991; R1; CD1; CORTICAL THYMOCYTE ANTIGEN CD1B; RP11-101J8.4

P29016

CD1B

Human Cells

A DNA sequence encoding the extracellular domain (Met1-Ser303) of human CD1B (NP_001755.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the CD1B/B2M heterodimer was purified.

Human

The recombinant heterodimer of human CD1B/B2M comprises 397 (297+100) amino acids and has a calculated molecular mass of 44.7 (33.0+ 11.7) KDa. The apparent molecular mass of rh CD1B/B2M heterodimer is approximately 39.6 and 13.1 KDa respectively in SDS-PAGE under reducing conditions.

(70.1+28.3) % as determined by SDS-PAGE