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Product Name
Sus scrofa (Pig) Legumain (His Tag) recombinant protein
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Description
The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with a strict specificity for hydrolysis of asparaginyl bonds. Known previously only from plants and invertebrates, Legumain is discovered as a lysosomal endopeptidase in mammals. Mammalian Legumain is a cysteine endopeptidase, inhibited by iodoacetamide and maleimides, but unaffected by compound E64. The Mammalian Legumain is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Legumain has been observed to be highly expressed in several types of solid tumors. It was demonstrated in membrane-associated vesicles concentrated at the invadopodia of tumor cells and on cell surfaces where it colocalized with integrins. Legumain was demonstrated to activate progelatinase A. Cells overexpressing Legumain possessed increased migratory and invasive activity in vitro and adopted an invasive and metastatic phenotype in vivo, inferring significance of Legumain in tumor invasion and metastasis. In addition, Legumain is expressed in both murine and human atherosclerotic lesions. The macrophage-specific expression of Legumain in vivo and ability of Legumain to induce chemotaxis of monocytes and endothelial cells in vitro suggest that Legumain may play a functional role in atherogenesis.
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Protein short names
AEP; PRSC1; AU022324; LGMN1; LEGUMAIN; AI746452; LGMN
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Uniprot ID
F1SD70
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the sus scrofa(Pig) LGMN (XP_001927117.4) (Met1-Tyr433) was expressed with a polyhistidine tag at the C-terminus.
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Protein Species
Sus scrofa (Pig)
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Molecular weight
The recombinant sus scrofa (Pig) LGMN consists of 427 amino acids and has a predicted molecular mass of 48.8 kDa. The apparent molecular mass of it is approximately 53.7 kDa in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Sus scrofa (Pig) Legumain / LGMN / AEP Protein (His Tag) SDS-PAGE
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