Suc-Ala-Phe-Lys-AMC peptide

Suc-Ala-Phe-Lys-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Suc-AFK-AMC

Suc-Ala-Phe-Lys-AMC

3

95.97%

C₃₂H₃₉N₅O₈

621.68

73207-91-7

71308635

Synthetic

Powder

The peptide Suc-Ala-Phe-Lys-AMC (succinyl-alanyl-phenylalanyl-lysyl-amidomethylcoumarin) is a fluorogenic peptide substrate that has been developed for the assay of plasmin. Plasmin is a trypsin-like enzyme involved in the dissolution of blood clots. Suc-Ala-Phe-Lys-AMC is a highly sensitive and specific plasmin substrate, and its use allows for the assay of both plasminogen activator and plasmin activity in the same cell fluids.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Pierzchala PA, Dorn CP, Zimmerman M. A new fluorogenic substrate for plasmin. Biochem J. 1979 Dec 1;183(3):555-9. doi: 10.1042/bj1830555. PMID: 161507; PMCID: PMC1161636.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.