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Product Name
E. coli ssPA/Stringent starvation protein A (His Tag)
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Description
Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme. It is synthesized predominantly when cells are exposed to amino acid starvation, at which time it accounts for over 50% of the total protein synthesized. It is involved in the transition from P1 early to P1 late gene expression. Rnk and SspA can functionally replace P.aeruginosa alginate regulatory gene algR2.
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Protein name
Stringent starvation protein A
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Protein short names
Y75_P3149
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Uniprot ID
P0ACA3
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Gene Name
sspA; pog; ssp; b3229; JW3198
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the E.Coli ssPA (P0ACA3) (Met 1-Ser 212) was expressed, with a polyhistidine tag at the N-terminus.
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Protein Species
E. coli
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Molecular weight
The recombinant E.Coli ssPA consisting of 228 amino acids and has a calculated molecular mass of 26.4 kDa. It migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Validations
Escherichia coli ssPA / Stringent starvation protein A Protein (His Tag) SDS-PAGE
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