-
Product Name
ST8SIA1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ST8SIA1
-
Tested applications
WB
-
Species reactivity
Human
-
Alternative names
ST8SIA1 antibody; GD3S antibody; SIAT8 antibody; SIAT8-A antibody; SIAT8A antibody; ST8SiaI antibody; alpha-N-acetylneuraminide alpha-2,8-sialyltransferase antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 49-270 of human ST8SIA1 (NP_003025.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - ST8SIA1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ST8SIA1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"