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Product Name
Recombinant SPC25 protein (His tag)
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Description
Recombinant Human Kinetochore protein Spc25 (hSpc25) fragment (amino acids 1-224) is a high-purity, stable protein designed for structural and biochemical studies. This fragment encompasses a computationally optimized soluble domain, ideal for investigating kinetochore assembly, microtubule interactions, and mitotic regulation.
The full-length Spc25 protein is a critical component of the NDC80 complex, essential for accurate chromosome segregation and spindle checkpoint activity during cell division. It localizes to the outer plate of the kinetochore from late prophase to anaphase, where it helps maintain kinetochore integrity and organizes microtubule-binding sites. The NDC80 complex, including Spc25, synergistically enhances microtubule affinity with the SKA1 complex, enabling tracking of depolymerizing microtubules and ensuring proper chromosome alignment and separation.
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Protein name
Kinetochore protein Spc25
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Uniprot ID
Q9HBM1
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Gene Name
SPC25; SPBC25
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-224 aa (Q9HBM1) were fused with C-terminal 6His tag.
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Sequence
MVEDELALFD KSINEFWNKF KSTDTSCQMA GLRDTYKDSI KAFAEKLSVK LKEEERMVEM FLEYQNQISR QNKLIQEKKD NLLKLIAEVK GKKQELEVLT ANIQDLKEEY SRKKETISTA NKANAERLKR LQKSADLYKD RLGLEIRKIY GEKLQFIFTN IDPKNPESPF MFSLHLNEAR DYEVSDSAPH LEGLAEFQEN VRKTNNFSAF LANVRKAFTA TVYNLEHHHH HH
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 30.44 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 31 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant SPC25 protein (His tag) SDS-PAGE
M: Marker; Lane 1: SPC25; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"