SOR-C27 peptide

SOR-C27 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-EGKLSSNDTEGGLCKEFLHPSKVDLPR-OH

H-Glu-Gly-Lys-Leu-Ser-Ser-Asn-Asp-Thr-Glu-Gly-Gly-Leu-Cys-Lys-Glu-Phe-Leu-His-Pro-Ser-Lys-Val-Asp-Leu-Pro-Arg-OH

27

95.31%

C₁₂₇H₂₀₆N₃₆O₄₃S

2957.26

Synthetic

Powder

The inhibitory TRPV6-binding peptides SOR-C13 and SOR-C27 are derived from of the C-terminus of soricidin (accession number P0C2P6), a paralytic peptide from saliva of Northern Short-tailed shrew Blarina brevicauda. SOR-C13 and SOR-C27 bind to and inhibit calcium influx through TRPV6 expressed in HEK-293 cells with EC50 values of 14 nM and 65 nM respectively.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Xue H, Wang Y, MacCormack TJ, Lutes T, Rice C, Davey M, et al. Inhibition of Transient Receptor Potential Vanilloid 6 channel, elevated in human ovarian cancers, reduces tumour growth in a xenograft model. J Cancer. 2018;9(17):3196-3207

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.