-
Product Name
Recombinant SMD3 protein (His tag)
- Documents
-
Description
Recombinant Human Small nuclear ribonucleoprotein Sm D3 (Sm-D3) Fragment (aa 2-126) - A high-purity protein fragment encompassing the complete Sm functional domain, ideal for studying spliceosome assembly and pre-mRNA splicing mechanisms in biochemical and structural research applications.
Small nuclear ribonucleoprotein Sm D3 (SNRPD3) serves as a core component of multiple spliceosomal snRNPs (U1, U2, U4, U5), forming the essential building blocks of the spliceosome machinery. This protein plays critical roles in both major and minor spliceosome complexes, participating in pre-mRNA splicing through its involvement in the B complex (pre-catalytic spliceosome) and C complex (activated spliceosome). Additionally, Sm-D3 contributes to U12-type intron splicing via the minor spliceosome and participates in histone pre-mRNA 3'-end processing as part of the U7 snRNP. The protein exhibits dynamic subcellular localization, with SMN-mediated assembly into core snRNPs occurring in the cytosol before transport t
-
Protein name
Small nuclear ribonucleoprotein Sm D3
-
Uniprot ID
P62318
-
Gene Name
SNRPD3
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
DNA encoding 2-126 aa (P62318) were fused with C-terminal 6His tag.
-
Sequence
MNHKVSIGVP IKVLHEAEGH IVTCETNTGE VYRGKLIEAE DNMNCQMSNI TVTYRDGRVA QLEQVYIRGS KIRFLILPDM LKNAPMLKSM KNKNQGSGAG RGKAAILKAQ VAARGRGRGM GRGNIFQKRR LEHHHHHH
-
Protein Species
Human
-
Molecular weight
Predictes a molecular mass of 15.44 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 15 kDa band.
-
Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
-
Activity
Not tested.
-
Validations
Recombinant SMD3 protein (His tag) SDS-PAGE
M: Marker; Lane 1: SMD3; Lane 2: BSA
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"