SlRGF9 peptide

SlRGF9 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

D{YSO3}TPARKKP{Hyp}IHN-NH2

Asp-O-sulfo-L-tyrosine-Thr-Pro-Ala-Arg-Lys-Lys-Pro-L-Hydroxyproline-Ile-His-Asn-NH2

13

95.9%

C₆₉H₁₁₀N₂₂O₂₂S

1631.838

Synthetic

Powder

SlRGF9 is a tyrosine-sulfated small secreted peptide belonging to the RGF/GLV peptide family in tomato. Its coding gene is specifically transcribed in tapetal cells and microspore mother cells of anthers upon low-temperature induction during reproductive development. Functionally redundant with SlRGF10, it acts as an extracellular ligand after post-translational tyrosine sulfation; mature sulfated SlRGF9 binds the plasma membrane receptor complex SlRGFR6-SlSERK to trigger downstream calcium influx via SlCNGC16/18 channels, which corrects delayed programmed cell death (PCD) of cold-stressed tapetum and safeguards normal pollen maturation under chilling conditions. Single knockout of SlRGF9 barely impairs cold tolerance because endogenous SlRGF10 compensates its physiological function, while simultaneous deletion of both SlRGF9 and SlRGF10 leads to severe pollen abortion and dramatic yield loss under low temperature. Exogenous application of purified sulfated SlRGF9 alone fully rescues the cold-sensitive phenotype of the double-knockout mutant. Non-sulfated SlRGF9 lacks receptor-binding affinity and shows no anti-cold bioactivity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Chen, S., Zou, Y., Cui, H. et al. Cold-induced peptide signalling secures pollen resilience and crop yield. Nature (2026). https://doi.org/10.1038/s41586-026-10603-7

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.