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Product Name
M.viridifaciens M. viridifaciens NA (His Tag) recombinant protein
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Description
To release sialic acids for use as carbon and energy sources for this non-pathogenic bacterium while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors.
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Protein name
Sialidase
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Uniprot ID
Q02834
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Gene Name
nedA
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the M.viridifaciens neuraminidase (BAA00852.1) (Pro 42-Gly 403) was expressed, with a N-terminal polyhistidine tag.
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Protein Species
M.viridifaciens
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Molecular weight
The recombinant M.viridifaciens neuraminidase consisting of 369 amino acids and has a calculated molecular mass of 39.7 kDa as estimated in SDS-PAGE under reducing conditions as predicted.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is 10,000 pmoles/min/μg. as measured under the described conditions.
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Validations
M. viridifaciens Neuraminidase / Sialidase (Active) (His Tag) SDS-PAGE
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