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Product Name
Human RIOK1 / RIO kinase 1 (His & GST Tag) recombinant protein
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Description
RIOK1, also known as RIO kinase 1, is a member of the RIO family of atypical serine protein kinases first characterized in yeast. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. RIOK1 functios as a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. RioK1 and pICln bind to PRMT5 in a mutually exclusive fashion. This results in a PRMT5-WD45/MEP50 core structure that either associates with pICln or RioK1 RIOK1 in distinct complexes. RIOK1 functions in analogy to pICln as an adapter protein by recruiting the RNA-binding protein nucleolin to the PRMT5 complex for its symmetrical methylation.
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Protein name
Serine/threonine-protein kinase RIO1
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Protein short names
AD034; BA288G3.1; RIO KINASE 1 (YEAST); SERINE/THREONINE-PROTEIN KINASE RIO1; 5430416A05RIK; RRP10; RIOK1; RP11-288G3.1; RIO KINASE 1; 3110046C13RIK
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Uniprot ID
Q9BRS2
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Gene Name
RIOK1
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human RIOK1 (Q9BRS2) (Met1-Lys568) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human RIOK1/GST chimera consists of 805 amino acids and has a calculated molecular mass of 93.4 kDa. The recombinant protein migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
Kinase activity untested
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Validations
Human RIOK1 / RIO kinase 1 Protein (His & GST Tag) SDS-PAGE
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