Mouse CHK2/CHEK2 (His & GST Tag) recombinant protein

Cat.#: 503033

Size:

Special Price 442.8 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Mouse CHK2/CHEK2 (His & GST Tag) recombinant protein
  • Documents
  • Description
    Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.
  • Protein name
    Serine/threonine-protein kinase Chk2
  • Protein short names
    PP1425; RP11-436C9.1; HCDS1; CHEK2; LFS2; CDS1; CHK2; RAD53; HUCDS1
  • Uniprot ID
    Q9Z265
  • Gene Name
    Chek2; Chk2; Rad53
  • Source/Expression Host
    Baculovirus-Insect Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the mouse CHEK2 (Q9Z265) (Mey 1-Leu 546) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
  • Protein Species
    Mouse
  • Molecular weight
    The secreted recombinant mouse CHEK2/GST chimera consists of 783 amino acids and has a calculated molecular mass of 89 kDa. The recombinant protein migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Activity
    Kinase activity untested
  • Validations

    Mouse CHK2 / CHEK2 Protein (His & GST Tag) SDS-PAGE

    Mouse CHK2 / CHEK2 Protein (His & GST Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"