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Product Name
SEPT7 Polyclonal Antibody
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Description
Polyclonal antibody to SEPT7
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SEPT7 antibody; CDC10 antibody; CDC3 antibody; NBLA02942 antibody; SEPT7A antibody; septin-7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 307-436 of human SEPT7 (NP_001011553.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - SEPT7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SEPT7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - SEPT7 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using SEPT7 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SEPT7 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse Intestine using SEPT7 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SEPT7 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using SEPT7 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SEPT7 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using SEPT7 antibody .
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Background
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPT12, SEPT6, SEPT2 and probably SEPT4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"