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Product Name
SEMA4F Polyclonal Antibody
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Description
Polyclonal antibody to SEMA4F
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
SEMA4F antibody; M-SEMA antibody; PRO2353 antibody; S4F antibody; SEMAM antibody; SEMAW antibody; m-Sema-M antibody; semaphorin-4F antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 280-420 of human SEMA4F (NP_004254.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - SEMA4F Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SEMA4F antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Has growth cone collapse activity against retinal ganglion-cell axons.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"