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Product Name
SEMA4C Polyclonal Antibody
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Description
Polyclonal antibody to SEMA4C
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
SEMA4C antibody; M-SEMA-F antibody; SEMACL1 antibody; SEMAF antibody; SEMAI antibody; semaphorin-4C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 749-833 of human SEMA4C (NP_060259.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - SEMA4C Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SEMA4C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SEMA4C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using SEMA4C antibody at dilution of 1:100 (40x lens).
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Background
Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"