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Product Name
SEMA3A Polyclonal Antibody
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Description
Polyclonal antibody to SEMA3A
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
SEMA3A antibody; COLL1 antibody; HH16 antibody; Hsema-I antibody; Hsema-III antibody; SEMA1 antibody; SEMAD antibody; SEMAIII antibody; SEMAL antibody; SemD antibody; coll-1 antibody; semaphorin-3A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human SEMA3A (NP_006071.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - SEMA3A Polyclonal Antibody
Western blot analysis of extracts of mouse brain, using SEMA3A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"