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Product Name
SELENBP1 Polyclonal Antibody
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Description
Polyclonal antibody to SELENBP1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
SELENBP1 antibody; HEL-S-134P antibody; LPSB antibody; SBP56 antibody; SP56 antibody; hSBP antibody; selenium-binding protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SELENBP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SELENBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Western blot - SELENBP1 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and SELENBP1 knockout HeLa cells, using SELENBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - SELENBP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using SELENBP1 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SELENBP1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using SELENBP1 antibody . Blue: DAPI for nuclear staining.
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Background
Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria. Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"