Scolopendin 2 peptide

Scolopendin 2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

AGLQFPVGRIGRLLRK

Ala-Gly-Leu-Gln-Phe-Pro-Val-Gly-Arg-Ile-Gly-Arg-Leu-Leu-Arg-Lys

16

97.8%

Synthetic

Powder

Active against G+ E. faecium ATCC 19434, S. aureus ATCC 25923, and MRSA (MIC 12.5-25 uM), G- bacteria E. coli O157 ATCC 43895, S. typhi ATCC 19430, P. aeruginosa ATCC 27853 (including three resistant strains) (MIC 6.3-12.5 uM), and fungi C. albicans ATCC 90028, C. parapsilosis ATCC 22019, T. beigelii KCTC 7707, and T. rubrum KCTC 6345 (MIC 6.3-12.5 uM). Scolopendin 2 was found in Chinese red-headed centipede Scolopendra subspinipes mutilans.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lee H, Hwang JS, Lee J, Kim JI, Lee DG. Scolopendin 2, a cationic antimicrobial peptide from centipede, and its membrane-active mechanism. Biochim Biophys Acta. 2015;1848(2):634-42.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.