Sclerosin peptide

Sclerosin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

TPALAVVTTVLPAAAVTTAKSV

Thr-Pro-Ala-Leu-Ala-Val-Val-Thr-Thr-Val-Leu-Pro-Ala-Ala-Ala-Val-Thr-Thr-Ala-Lys-Ser-Val

22

96%

Synthetic

Powder

Warning: this peptide contains non-natural amino acids although they are represented using the closest standard 20 amino acids. Specifically, T1, T8, T17, and T18 are dehydrated into Dhb. P12 is 3,4-dehydrated (Dhp). In total, five residues are dehydrated. Residue K20 is Dab (2,4-diaminobutyric acid). Finally, the acyl chain CH(3)-(CH(2))(6)-CH(OH)-CH(2)-CO is attached to the N-terminus. Thus, the entire sequence is CH(3)-(CH(2))(6)-CH(OH)-CH(2)-CO-Dhb-Pro-Ala-Leu/Ile-Ala-Val-Val-Dhb-Thr-Val-Leu/Ile-Dhp-Ala-Ala-Ala-Val-Dhb-Dhb-Ala-Dab-Ser-Val-OH. Note that residues 4 and 11 can be either L or I. Sclerosin has Antifungal, Antibacterial activity. The source of Sclerosin is Pseudomonas sp. strain DF41.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Berry CL, Brassinga AK, Donald LJ, Fernando WG, Loewen PC, De kievit TR. Chemical and biological characterization of sclerosin, an antifungal lipopeptide. Can J Microbiol. 2012;58(8):1027-34.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.