SAAP 148 peptide

SAAP 148 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-LKRVWKRVFKLLKRYWRQLKKPVR-NH2

Ac-Leu-Lys-Arg-Val-Trp-Lys-Arg-Val-Phe-Lys-Leu-Leu-Lys-Arg-Tyr-Trp-Arg-Gln-Leu-Lys-Lys-Pro-Val-Arg-NH2

24

95.27%

C₁₅₇H₂₆₁N₄₉O₂₇

3267.05

Synthetic

Powder

SAAP 148 is a synthetic peptide derived from LL-37 which is more stable to plasma proteases and more effective in eradicating bacteria than the parent peptide. In vitro, SAAP (synthetic antimicrobial and antibiofilm peptide) 148 kills MDR pathogens without inducing resistance, prevents biofilm formation, and eliminates established biofilms and persister cells. A single 4-hour treatment with SAAP 148 completely eradicates acute and established biofilm associated infections with methicillin resistant Staphylococcus aureus and MDR Acinetobacter baumannii from wounded ex vivo human skin and murine skin in vivo.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• de Breij et al (2018) The antimicrobial peptide SAAP-148 combats drug-resistant bacteria and biofilms. Science Translational Medicine 10 (423) DOI: 10.1126/scitranslmed.aan4044
• Scheper et al (2018) SAAP-148 Eradicates MRSA Persisters Within Mature Biofilm Models Simulating Prosthetic Joint Infection. Front Microbiol. 12 625952 PMID: 33584628
• van Gent et al (2022) Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin. Antibiotics (Basel) 11(5) 673 PMID: 35625317

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.