SAAP 148 peptide
Not For Human Use, Lab Use Only.
Cat.#: 319127
Special Price 203.9 USD
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Product Name
SAAP 148 peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
Ac-LKRVWKRVFKLLKRYWRQLKKPVR-NH2
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Sequence
Ac-Leu-Lys-Arg-Val-Trp-Lys-Arg-Val-Phe-Lys-Leu-Leu-Lys-Arg-Tyr-Trp-Arg-Gln-Leu-Lys-Lys-Pro-Val-Arg-NH2
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Length (aa)
24
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Peptide Purity (HPLC)
95.27%
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Molecular Formula
C157H261N49O27
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Molecular Weight
3267.05
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Source
Synthetic
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Form
Powder
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Description
SAAP 148 is a synthetic peptide derived from LL-37 which is more stable to plasma proteases and more effective in eradicating bacteria than the parent peptide. In vitro, SAAP (synthetic antimicrobial and antibiofilm peptide) 148 kills MDR pathogens without inducing resistance, prevents biofilm formation, and eliminates established biofilms and persister cells. A single 4-hour treatment with SAAP 148 completely eradicates acute and established biofilm associated infections with methicillin resistant Staphylococcus aureus and MDR Acinetobacter baumannii from wounded ex vivo human skin and murine skin in vivo.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- de Breij et al (2018) The antimicrobial peptide SAAP-148 combats drug-resistant bacteria and biofilms. Science Translational Medicine 10 (423) DOI: 10.1126/scitranslmed.aan4044
- Scheper et al (2018) SAAP-148 Eradicates MRSA Persisters Within Mature Biofilm Models Simulating Prosthetic Joint Infection. Front Microbiol. 12 625952 PMID: 33584628
- van Gent et al (2022) Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin. Antibiotics (Basel) 11(5) 673 PMID: 35625317
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"