RW4 peptide
Not For Human Use, Lab Use Only.
Cat.#: 319126
Special Price 117.7 USD
-
Product Name
RW4 peptide
-
Documents
Batch to batch variation of the purity
-
Sequence Shortening
H-RWRWRWRW-NH2
-
Sequence
H-Arg-Trp-Arg-Trp-Arg-Trp-Arg-Trp-NH2
-
Length (aa)
8
-
Peptide Purity (HPLC)
95.08%
-
Molecular Formula
C68H91N25O8
-
Molecular Weight
1386.61
-
Source
Synthetic
-
Form
Powder
-
Description
The RW4 peptide, part of the (RW)n series, is a synthetic cationic antimicrobial peptide composed of four repeats of arginine (R) and tryptophan (W) residues, with a C-terminal amidation. This peptide exhibits a strong interaction with negatively charged phospholipid bilayers, such as those containing phosphatidylglycerol (POPG), as demonstrated by circular dichroism spectroscopy and tryptophan fluorescence studies. The RW4 peptide shows enhanced antimicrobial activity with increasing chain length, attributed to its ability to partition into lipid membranes and disrupt bilayer integrity. Spectroscopic data indicate that RW4 undergoes conformational changes upon binding to lipid vesicles, suggesting a mechanism involving membrane intercalation.
Fluorescence studies reveal that the tryptophan residues in RW4 are more protected in POPG vesicles compared to zwitterionic phosphatidylcholine (POPC) vesicles, highlighting its preferential interaction with negatively charged membranes. The peptide's antimicrobial efficacy correlates with its chain length, with longer peptides like RW4 demonstrating greater activity. These findings underscore the importance of arginine and tryptophan residues in mediating peptide-lipid interactions and membrane disruption, providing insights into the design of short, potent antimicrobial peptides.
-
Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
-
References
- Strøm et al (2002) Antimicrobial activity of short arginine- and tryptophan-rich peptides. J. Pept. Sci. 8(8) 431 PMID: 12212806
- Liu et al (2007) Length effects in antimicrobial peptides of the (RW)n series. Antimicrob. Agents Chemother. 51(2) 597 PMID: 17145799
- Mohan et al (2014) Enhanced antimicrobial activity of peptide-cocktails against common bacterial contaminants of ex vivo stored platelets. Clin. Microbiol. Infect. 20(1) O39 PMID: 23926880
-
About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
-
Molar Concentration Calculator
-
Dilution Calculator
-
Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"