Rusalatide peptide

Rusalatide peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-AGYKPDEGKRGDACEGDSGGPFV-NH2

H-Ala-Gly-Tyr-Lys-Pro-Asp-Glu-Gly-Lys-Arg-Gly-Asp-Ala-Cys-Glu-Gly-Asp-Ser-Gly-Gly-Pro-Phe-Val-NH2

23

95.45%

C₉₇H₁₄₇N₂₉O₃₅S

2311.43

875455-82-6

91668177

Synthetic

Powder

Rusalatide (TP508) is a 23 amino acid peptide representing amino acids 508-530 of human prothrombin and was identified as the high - affinity binding domain of thrombin responsible for interaction with a subset of thrombin receptors on the surface of fibroblasts thought to initiate tissue repair. Its specificity has been demonstrated in both in vitro and in vivo experiments by altering the sequence and/or using scrambled peptides. TP508 was shown to initiate tissue repair and regeneration by reversing endothelial dysfunction, stimulating revascularization, attenuating inflammation and reducing apoptosis.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Kantara C, Moya SM, Houchen CW, Umar S, Ullrich RL, Singh P, Carney DH. Novel regenerative peptide TP508 mitigates radiation-induced gastrointestinal damage by activating stem cells and preserving crypt integrity. Lab Invest. 2015 Nov;95(11):1222-33. doi: 10.1038/labinvest.2015.103. Epub 2015 Aug 17. PMID: 26280221; PMCID: PMC4626368.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.