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Product Name
RSAD2 antibody
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Description
RSAD2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human skin tissue, human osteosarcoma tissue. Positive WB detected in A549 cells, mouse lung tissue, Raji cells. Observed molecular weight by Western-blot: 43-48 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
2510004L01Rik antibody; cig33 antibody; cig5 antibody; RSAD2 antibody; vig1 antibody; Viperin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RSAD2 recombinant protein (Accession Number: XM_011510414). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:114933(RSAD2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human skin using Catalog No:114933(RSAD2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:114933(RSAD2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RSAD2 antibody Catalog No:114933 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon. Western blot analysis of IFNG-treated cells revealed 43 kD/35kd bands.
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References
- Gielen V, Johnston SL, Edwards MR. Azithromycin induces anti-viral responses in bronchial epithelial cells. The European respiratory journal. 36(3):646-54. 2010.
- Schmeisser H, Mejido J, Balinsky CA. Identification of alpha interferon-induced genes associated with antiviral activity in Daudi cells and characterization of IFIT3 as a novel antiviral gene. Journal of virology. 84(20):10671-80. 2010.
- Ru J, Sun H, Fan H. MiR-23a facilitates the replication of HSV-1 through the suppression of interferon regulatory factor 1. PloS one. 9(12):e114021. 2014.
- Lubick KJ, Robertson SJ, McNally KL. Flavivirus Antagonism of Type I Interferon Signaling Reveals Prolidase as a Regulator of IFNAR1 Surface Expression. Cell host & microbe. 18(1):61-74. 2015.
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