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RNF156 antibody

Cat.#: 114744

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Product Information

  • Product Name
    RNF156 antibody
  • Documents
  • Description
    RNF156 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells, HepG2 cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 66-72 kDa,55 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    KIAA0544 antibody; mahogunin antibody; ring finger 1 antibody; MGRN1 antibody; RING finger protein 156 antibody; RNF156 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RNF156 recombinant protein (Accession Number: BC014199). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114744(MGRN1 antibody) at dilution of 1:500

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114744(MGRN1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114744(MGRN1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114744(MGRN1 antibody) at dilution of 1:100 (under 10x lens)

  • Background
    MGRN1(E3 ubiquitin-protein ligase) mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. It also plays a role in the regulation of endosome-to-lysosome trafficking. Human and mouse mahogunin exhibit a similar expression pattern across multiple tissues with particularly high levels in brain(PMID: 12560552).It has 4 isoforms produced by alternative splicing.
  • References
    • Overton JD, Leibel RL. Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein. The Journal of biological chemistry. 286(21):18914-29. 2011.
    • Gunn TM, Silvius D, Bagher P, Sun K, Walker KK. MGRN1-dependent pigment-type switching requires its ubiquitination activity but not its interaction with TSG101 or NEDD4. Pigment cell & melanoma research. 26(2):263-8. 2013.
    • Jiao J, Sun K, Walker WP, Bagher P, Cota CD, Gunn TM. Abnormal regulation of TSG101 in mice with spongiform neurodegeneration. Biochimica et biophysica acta. 1792(10):1027-35. 2009.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"