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Product Name
RNF151 antibody
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Description
RNF151 Rabbit Polyclonal antibody. Positive WB detected in rat testis tissue, human testis tissue, mouse testis tissue. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 26 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ring finger protein 151 antibody; RNF151 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNF151 recombinant protein (Accession Number: NM_174903). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
rat testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114743(RNF151 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:114743(RNF151 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:114743(RNF151 Antibody) at dilution of 1:50 (under 40x lens)
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Background
RNF151 also termed as ring finger protein 151 is a 245 amino acid protein, which contains one Ring-type zinc finger and one TRAF-type zinc finger. RNF151, with, a putative nuclear localization signal (NLS)was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII–IX seminiferous tubules.
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