RNF149 Polyclonal Antibody

Cat.#: 162741

Special Price 174.2 USD

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Product Information

  • Product Name
    RNF149 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to RNF149
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    RNF149 antibody; DNAPTP2 antibody; ring finger protein 149 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 221-400 of human RNF149 (NP_775918.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - RNF149 Polyclonal Antibody

    Western blot - RNF149 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using RNF149 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

  • Background
    E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"