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Product Name
RNF133 antibody
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Description
RNF133 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HL-60 cells, K-562 cells. Positive IF detected in Hela. Positive IHC detected in human testis. Observed molecular weight by Western-blot: 45-48kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
RNF133 antibody; ring finger protein 133 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNF133 recombinant protein (Accession Number: NM_139175). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114739(RNF133 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:114739(RNF133 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:114739(RNF133 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RNF133 antibody Catalog No:114739 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RNF133 represents ring finger protein 133
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"