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Product Name
Recombinant RIFK protein (His tag)
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Description
This product is a recombinant fragment of human Riboflavin kinase (RFK), spanning residues 1-155, designed for structural and biochemical studies. It represents a stable and soluble unit of the enzyme, which catalyzes the phosphorylation of riboflavin to form flavin mononucleotide (FMN), a critical step in flavin adenine dinucleotide (FAD) biosynthesis.
Riboflavin kinase (EC 2.7.1.26), encoded by the RFK gene in humans, functions as an ATP:riboflavin 5'-phosphotransferase to produce FMN, making it the rate-limiting enzyme in FAD synthesis. It plays an essential role in TNF-induced reactive oxygen species (ROS) production by physically and functionally coupling TNFRSF1A to NADPH oxidase through interactions with both TNFRSF1A and CYBA. This coupling enhances FAD incorporation into NADPH oxidase, which is crucial for its assembly and activation. The protein is localized in the cytoplasm, underscoring its involvement in intracellular signaling and metabolic pathways.
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Protein name
Riboflavin kinase
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Uniprot ID
Q969G6
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Gene Name
RFK
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-155 aa (Q969G6) were fused with C-terminal 6His tag.
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Sequence
MRHLPYFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DISTGIYYGW ASVGSGDVHK MVVSIGWNPY YKNTKKSMET HIMHTFKEDF YGEILNVAIV GYLRPEKNFD SLESLISAIQ GDIEEAKKRL ELPEHLKIKE DNFFQVSKSK IMNGHLEHHH HHH
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 18.67 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 19 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant RIFK protein (His tag) SDS-PAGE
M: Marker; Lane 1: RIFK; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"