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Product Name
RHOB antibody
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Description
RHOB Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue, human gliomas tissue, human liver tissue. Positive IF detected in MCF-7 cells. Positive IP detected in HeLa cells. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ARH6 antibody; ARHB antibody; MST081 antibody; MSTP081 antibody; Rho cDNA clone 6 antibody; RHOB antibody; RHOH6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RHOB recombinant protein (Accession Number: NM_004040). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114658(RHOB antibody) at dilution of 1:2000
IP Result of anti-RHOB (IP:Catalog No:114658, 3ug; Detection:Catalog No:114658 1:2000) with HeLa cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:114658(RHOB Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:114658(RHOB Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using RHOB antibody Catalog No:114658 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RHOB represents ras homolog gene family, member B
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References
- Zhang C, Elkahloun AG, Liao H. Expression signatures of the lipid-based Akt inhibitors phosphatidylinositol ether lipid analogues in NSCLC cells. Molecular cancer therapeutics. 10(7):1137-48. 2011.
- Gao Y, Song C, Hui L. Overexpression of RNF146 in non-small cell lung cancer enhances proliferation and invasion of tumors through the Wnt/β-catenin signaling pathway. PloS one. 9(1):e85377. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"