Rhesus NKG2D / CD314 (aa 78-216, His Tag) recombinant protein

Rhesus NKG2D / CD314 (aa 78-216, His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.

NATURAL KILLER CELL GROUP 2D; NKG2-D TYPE II INTEGRAL MEMBRANE PROTEIN; KLRK1; KLR; NK CELL RECEPTOR D; D12S2489E; D6H12S2489E; NKG2D; CD314; NKG2-D-ACTIVATING NK RECEPTOR; NKG2-D

Q9MZJ7

Baculovirus-Insect Cells

A DNA sequence encoding the rhesus NKG2D (NP_001028061.1) (Phe78-Val216) was expressed with a polyhistide tag at the N-terminus.

Rhesus

The recombinant rhesus NKG2D consists of 155 amino acids and has a calculated molecular mass of 18.3 kDa.

> 95 % as determined by SDS-PAGE

1. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch (Cat:502650), The EC₅₀ of human ULBP1-Fch (Cat:502650) is 0.12-0.28 μg/ml.
2. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (Cat:503201), The EC₅₀ of human MICB-Fch (Cat:503201) is 33.8-79 ng/ml.